The software described in this document is furnished under a. However, the side length of its square pinhole is usually specified in airy disk units, referenced to a standard fixed wavelength of 580 nm, making it independent of the actual overall magnification. But the engineer has to find right version software for our system. Since the lcs software is based on the user administration of windows. Leica tcs sp2 leica tcs sp5 ii broadband confocal microscope leica tcs sted cw.
Abstract satellite cell sc transplantation represents a powerful strategy to investigate sc biology during muscle regeneration. The leica d flim an mp flim systems are addons of the tcs sp2 and tcs sp5 laser scanning microscopes. This activates only the air cooling of the argon laser. Furthermore, leica will cease to provide service for the sp2 within the next year. Leica tcs sp2 laser scanning spectral confocal microscope. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Uhco technical services university of houston college of. The fluorescence emission of 1 from cells was spectrally dispersed using a prism and detected using a pm tube. Basic training video for use of the leica confocal microscope at tufts. Open source free software for a leica tcs sp5 confocal. The operating software offers frap and fret with built in wizards to help. This leica sp2 platform is equipped with two leica confocal microscopes. Instructions for leica sp2 confocal equipped with visible. Leica confocal sp2 mpflim advanced light microscopy and.
True confocal scanner leica tcs sp2 universitetet i bergen. With the las x control software, leica microsystems offers an interface, which shows all you need to know about your confocal on one screen. Software license the software described in this document is furnished under a license agreement which is included with the product. Make sure that your sample slides are clean and sealed. The leica tcs sp2 is supplied with the latest version of the licensed leica confocal software. This agreement specifies the permitted and prohibited uses of the product. Along with many tiffs, the folder also contains a text description in the. Leica tcs 4d first fourdetector confocal for multidye applications first spectral detector in confocal microscopy leica tcs sp2 first confocal with excitation from uv to ir hc optics system ensures superlative optical performance first use of aotf for laser modulation in a confocal first dedicated objectives for confocal scanning cs. Drawingalineontheannotationsheet240 drawingarectangleontheannotationsheet240. The leica hc pl irapo objectives for example deliver superior broadband transmission that is higher than 85% from 470 to 1200 nm. To maintain information on the most current level, the description of software. Growth of desferrioxamine deficient streptomyces mutants through xenosiderophore piracy of airborne fungal contaminations. The basic software allows you to capture images from the microscope but the more advanced features will stay locked.
Leica sp8 confocal with white light laser system operates from a dmi8 cs bino inverted microscope with afc closed loop focusing, scanning and super zgalvo stages, turret cooling, spill protection, and cuda workstation motorized dic accessories. Application letters leica tcs sp2 and tcs sp2 aobs confocal express published 30 mars 2009 confocal microscopy, leica sp2. Pdf growth of desferrioxamine deficient streptomyces. We have used leica s tcs sp2 laser scanning spectral confocal microscope for more than a year. Leica tcs sp8 confocal microscope system andrew lackner. Standard confocal microscopy with slides and nonstandard specimens, multiphoton microscopy and fluorescence lifetime imaging.
The leica lcs software can perform multidimensional image processing, fret, frap. Leica tcs sp2 rs, laser scanning spectral confocal microscope parts. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Leica tcs sp2 is a laser scanning confocal microscope with electronically. The online help system opens with an activated contents window. Imaging was performed using a confocal laser scanning microscope leica tcs sp2, coupled to a cw argonion laser 488 nm. Leica tcs sp2 confocal microscopes products home leica leica microsystems. Molecular rotor measures viscosity of live cells via. Software software for image tridimensional processing. This will create individual directories that can be viewed only by the respective user. Nikon eclipse e600 light epifluorescence microscope with several infinity objectives and differential interference contrast, lightfield, darkfield, and phase. Las af 3 intuitive software that makes your life easier 24 leica tcs sp8 x freely tunable excitation 26 leica tcs sp8 sted opening the gate to superresolution 28 leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars.
Employing a tunable aobs beam splitter, the tcs sp2 is capable of simultaneous 4 channel acquisition and utilizing a kscanner allows the system to achieve higher scan speeds and perform scan field rotation. Pdf dominant negative effects of a nonconducting trek1. To maintain information on the most current level, the description of software functions was intentionally. Inverted laser scanning confocal microscope system with solent temperature controlled surround for live cell work. Growth of desferrioxaminedeficient streptomyces mutants. Leica sp2 experiments are saved as multiple tiff files in a single folder. Samples were then examined under a leica tcs sp2 confocal laserscanning microscope at a wavelength of 488 nm for excitation and 530 nm green for emission. The leica tcs spes streamlined approach makes it accessible to every user, beginners and experts alike. Tcs software with 3d capacity dongle with the pc pc hp sw 6600 workstation. Leica confocal tcs sp2 this is part of the backprojected pinhole calculator. Dominant negative effects of a nonconducting trek1 splice variant expressed in brain. If you select yes, the stage is going to move to all 4 corners to calibrate the position of the stage. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions.
Hi ondrej, in the past spring april, 2014 we just upgraded sp5 ii from win xp to win7 with new computer. Pcmicroscope scanner power laser power laser key las af ok. Quantification of germinating spores at a distance from the fungus was performed using the 3d object counter tool of the imagejfiji software schindelin et al. Image handling and analysis is accomplished using nihimage software.
Confocal microscopy is done on a leica tcs sp2 confocal microscope with image pro software. A macintosh power mac 7300180 is connected to an image capture camera mounted on an olympus bh2 binocular microscope. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. It is recommended setting up a separate user id for each user system administrator.
We described here a protocol for sc isolation from green fluorescent protein gfpexpressing mice and their transplantation into murine muscles. Leica sp5 training manual september 2010 for the argon laser, adjust. Application letters leica tcs sp2 and tcs sp2 aobs. To retrieve the contents of the online help system. Center for metals in biological systems cmbs duquesne. Please browse the internet to learn interactively all about. Statistical analysis statistical calculations were performed with medcalc 9. The position of the focus plane at different wavelengths shows almost no deviation over a wide range of wavelengths. Tcs sp2 laser scanning spectral confocal microscope from leica. Lifetime upgrade kit for leica tcs sp2 fluorescence lifetime imaging with ps resolution ultrahigh sensitivity due to tcspc technique zoom and image rotation functions of tcs sp2 applicable fret experiments fluorescence quenching autofluorescence separation of chromophores by lifetime autofluorescence of a nematode. Leica tcs sp5 confocal laser scanning microscope user guide. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence.
To always keep the information up to date, the description of software functions was intentionally omitted from these operating instructions. To join, leave or search the confocal microscopy listserv, go to. Leica tcs sp ii comes with a context sensitive online help system that teaches you the different functions of the system. Leica tcs sp2 spectral confocal microscope system and computer control workstation hp x4000, dual screens, software and offline workstation.
Leica confocal software lcs complements the optics and electronics by. Lifetime upgrade kit for leica tcs sp2 photonic solutions. Ive found that getting micromanager to work with leica cameras was a bit of a pain. Beckman institute for advanced science and technology. Leica tcs sp2 switch on the lumen 200 fluorescence illumination system switch on the pc red rocker switch main on the desk a. To learn about the leica confocal software, use the book as a. Switch on the confocal scanner red rocker switch b.
Please contact your sales person with possible substitutions. I found out recently through a similar situation that if you have an las installer, the software will work even without a software keydongle. The leica tcs sp2 is a point scanning confocal microscope, based around an. Calculated axial chromatic aberration for hc pl apo 100x1. This handbook should be considered a supplement to the handbooks of the leica tcs sp2, leica tcs sp5, and the bh tcspc. Leica confocal software, use the book as a tutorial. This will activate the pc, the monitors, the microscope controller and blue hene laser.
Many pages are copied from internet web pages or from presentations given by leica, zeiss and other companies. Leica tcs sp2 confocal inverted mrc centre for regenerative. This can contain many different series acquired during the one experiment. The whole process takes one day and so far everything works great. Instead, you are referred to the online help of the leica application suite. A message will appear asking whether you want to initialize the stage. Dmire2 inverted microscope objectives hc pl apo cs 10x0,30 dry 11 mm. Details the leica tcs sp2 has a system magnification of 3. This manual is the first section of a three part leica tcs sp5 user guide edited by. Confocal microscopy list leica tcs sp2, lcs and win7. Introduction to confocal laser scanning microscopy leica this presentation has been put together as a common effort of urs ziegler, anne greet bittermann, mathias hoechli.
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